Genotyping-by-sequencing

Genotyping-by-sequencing

IGA is using different methods of genotyping-by-sequencing (GBS) for the construction of high density genetic maps and genetic diversity analysis. This unprecedented marker saturation opens the door to new applications in plant breeding, genome analysis and trait dissection.

These procedures can be applied to any plant species, even in the absence of a reference genome. IGA-subsidiary IGATech uses this know-how to offer a service of GBS and genetic mapping to breeding companies and plant scientists. See the application of this technology to two case studies: kiwifruit and the huge 16 gigabase genome of Cedrus atlantica.

Scaglione et al (2015) A RAD-based linkage map of kiwifruit (Actinidia chinensis Pl.) as a tool to improve the genome assembly and to scan the genomic region of the gender determinant for the marker-assisted breeding. Tree Genetics & Genomes 11: 115

Karam et al (2015) Genomic exploration and molecular marker development in a large and complex conifer genome using RADseq and mRNAseq. Molecular Ecology Resources 15:601-612